Hotspot

rps2

LSC · 15348-16184 · 837 bp

Back to Semiaquilegia

Pi 0.0131

MarkerSeek score 66.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 837
variable_sites: 11
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ATTGTTCGGATACCCAGTCA	TTTAACTAGCGGGTCCGTTC	45.0	50.0	56.2	57.6	988-993 bp	1.0	63.9
rps2_p2	TGTTCGGATACCCAGTCATT	TTTAACTAGCGGGTCCGTTC	45.0	50.0	56.2	57.6	986-991 bp	1.0	63.9
rps2_p3	TTGTTCGGATACCCAGTCAT	TTTAACTAGCGGGTCCGTTC	45.0	50.0	56.2	57.6	987-992 bp	1.0	63.9
rps2_p4	TGTTCGGATACCCAGTCATT	TAACTAGCGGGTCCGTTCTT	45.0	50.0	56.2	58.5	984-989 bp	1.0	63.2
rps2_p5	TGTTCGGATACCCAGTCATT	TTAACTAGCGGGTCCGTTCT	45.0	50.0	56.2	58.5	985-990 bp	1.0	63.2