Hotspot

rpl2

IRa · 160663-162141 · 1479 bp

Back to Semiaquilegia

Pi NA

MarkerSeek score 35.70

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1479
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	ACTTAAACTTAATAACACGGCA	GTGAACGTGTCACGGCTAAT	31.8	50.0	54.1	58.6	1579 bp	0.5	20.0
rpl2_p2	AACTTAAACTTAATAACACGGCA	GTGAACGTGTCACGGCTAAT	30.4	50.0	54.8	58.6	1580 bp	0.5	20.0
rpl2_p3	ACTTAAACTTAATAACACGGCA	GTGAACGTGTCACGGCTAA	31.8	52.6	54.1	58.1	1579 bp	0.5	20.0
rpl2_p4	AACTTAAACTTAATAACACGGCA	GTGAACGTGTCACGGCTAA	30.4	52.6	54.8	58.1	1580 bp	0.5	20.0
rpl2_p5	CTTAAACTTAATAACACGGCA	GTGAACGTGTCACGGCTAAT	33.3	50.0	52.2	58.6	1578 bp	0.5	20.0