Hotspot

ycf1-trnR-ACG

IRa · 135610-136986 · 1377 bp

Back to Semiaquilegia

Pi 0.00957

MarkerSeek score 65.60

Primer available yes

Label ycf1-trnR-ACG

Feature Metadata

label_name: ycf1-trnR-ACG
feature_type: igs
region: IRa
length_bp: 1377
variable_sites: 13
pi: 0.00957
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnR-ACG_p1	AAAACCACGACAAACCTCCT	CAGAGGATTAGAGCACGTGG	45.0	55.0	57.6	57.8	1510-1521 bp	1.0	76.5
ycf1-trnR-ACG_p2	AAACCACGACAAACCTCCTT	CAGAGGATTAGAGCACGTGG	45.0	55.0	57.6	57.8	1509-1520 bp	1.0	76.5
ycf1-trnR-ACG_p3	CAAAACCACGACAAACCTCC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.5	57.8	1511-1522 bp	1.0	76.1
ycf1-trnR-ACG_p4	AAAACCACGACAAACCTCCT	GGCCTGTAGCTCAGAGGATT	45.0	55.0	57.6	58.9	1521-1532 bp	1.0	71.3
ycf1-trnR-ACG_p5	AAACCACGACAAACCTCCTT	GGCCTGTAGCTCAGAGGATT	45.0	55.0	57.6	58.9	1520-1531 bp	1.0	71.3