Hotspot

trnN-GUU-ycf1

IRb · 115221-115928 · 708 bp

Back to Semiaquilegia

Pi 0.0174

MarkerSeek score 71.30

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 708
variable_sites: 12
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	ATCTCCCCAGGTAGGATTCG	AAAACCACGACAAACCTCCT	55.0	45.0	58.0	57.6	853-864 bp	1.0	79.3
trnN-GUU-ycf1_p2	ATCTCCCCAGGTAGGATTCG	AAACCACGACAAACCTCCTT	55.0	45.0	58.0	57.6	852-863 bp	1.0	79.3
trnN-GUU-ycf1_p3	ATCTCCCCAGGTAGGATTCG	CAAAACCACGACAAACCTCC	55.0	50.0	58.0	57.5	854-865 bp	1.0	78.9
trnN-GUU-ycf1_p4	AGGATTCGAACCTACGACCA	AAAACCACGACAAACCTCCT	50.0	45.0	58.2	57.6	841-852 bp	1.0	78.2
trnN-GUU-ycf1_p5	AGGATTCGAACCTACGACCA	AAACCACGACAAACCTCCTT	50.0	45.0	58.2	57.6	840-851 bp	1.0	78.2