Hotspot

trnN-GUU-infA

LSC · 85309-85736 · 428 bp

Back to Semiaquilegia

Pi 0.0308
MarkerSeek score 74.20
Primer available yes
Label trnN-GUU-infA

Feature Metadata

label_name
trnN-GUU-infA
feature_type
igs
region
LSC
length_bp
428
variable_sites
13
pi
0.0308
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-infA_p1 CCGTTGTTCCTCAGTAGCTC GTTATGCTTCGGGGAGGATC 55.0 55.0 58.0 57.8 590-645 bp 1.0 82.2
trnN-GUU-infA_p2 ATTTGCCGTTGTTCCTCAGT GTTATGCTTCGGGGAGGATC 45.0 55.0 58.0 57.8 595-650 bp 1.0 82.1
trnN-GUU-infA_p3 CGAATTTGCCGTTGTTCCTC GTTATGCTTCGGGGAGGATC 50.0 55.0 58.0 57.8 598-653 bp 1.0 81.9
trnN-GUU-infA_p4 TGGGTTGAGAACGGGAATTG GTTATGCTTCGGGGAGGATC 50.0 55.0 58.1 57.8 631-686 bp 1.0 81.3
trnN-GUU-infA_p5 TGGTCGTAGGTTCGAATCCT GTTATGCTTCGGGGAGGATC 50.0 55.0 58.2 57.8 541-596 bp 1.0 81.3