Hotspot

rpl36-trnN-GUU

LSC · 84527-85236 · 710 bp

Back to Semiaquilegia

Pi 0.016

MarkerSeek score 64.20

Primer available yes

Label rpl36-trnN-GUU

Feature Metadata

label_name: rpl36-trnN-GUU
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 10
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-trnN-GUU_p1	GTCTCTGTTTATGCCTCGGG	ATCTCCCCAGGTAGGATTCG	55.0	55.0	58.1	58.0	817-890 bp	1.0	84.3
rpl36-trnN-GUU_p2	TCTGTTTATGCCTCGGGTTG	ATCTCCCCAGGTAGGATTCG	50.0	55.0	57.9	58.0	814-887 bp	1.0	83.9
rpl36-trnN-GUU_p3	CCTCGGGTTGGAGCAAATTA	ATCTCCCCAGGTAGGATTCG	50.0	55.0	57.9	58.0	804-877 bp	1.0	83.8
rpl36-trnN-GUU_p4	ACGGATCAGTCGGCATTTTT	ATCTCCCCAGGTAGGATTCG	45.0	55.0	58.2	58.0	765-838 bp	1.0	83.4
rpl36-trnN-GUU_p5	TTTTACGAATGGAAGCCCGT	ATCTCCCCAGGTAGGATTCG	45.0	55.0	57.8	58.0	739-812 bp	1.0	83.4