Hotspot

rps12-clpP

LSC · 73518-76141 · 2624 bp

Back to Semiaquilegia

Pi 0.00894

MarkerSeek score 63.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 2624
variable_sites: 23
pi: 0.00894
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	AGAGCGGGGGATTTTGTTAC	TATGACACCAGAAGAAGCGC	50.0	50.0	57.9	58.0	2755-2789 bp	1.0	81.1
rps12-clpP_p2	GAGCGGGGGATTTTGTTACA	TATGACACCAGAAGAAGCGC	50.0	50.0	58.2	58.0	2754-2788 bp	1.0	80.7
rps12-clpP_p3	GAAGAGCGGGGGATTTTGTT	TATGACACCAGAAGAAGCGC	50.0	50.0	58.5	58.0	2757-2791 bp	1.0	78.4
rps12-clpP_p4	AGCGGGGGATTTTGTTACAT	TATGACACCAGAAGAAGCGC	45.0	50.0	57.5	58.0	2753-2787 bp	1.0	77.9
rps12-clpP_p5	AGAGCGGGGGATTTTGTTAC	CCACCCGATTCGGATGATTC	50.0	55.0	57.9	58.5	2692-2729 bp	1.0	77.3