Hotspot

ndhC-trnV-UAC

LSC · 51745-52715 · 971 bp

Back to Semiaquilegia

Pi 0.0372
MarkerSeek score 71.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
971
variable_sites
36
pi
0.0372
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1027-1050 bp 1.0 50.3
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1026-1049 bp 1.0 47.6
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1015-1038 bp 1.0 46.5
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.6 57.8 1026-1049 bp 1.0 46.3
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.6 57.8 1027-1050 bp 1.0 46.3