Hotspot

trnL-UAA-trnF-GAA

LSC · 49094-49444 · 351 bp

Back to Semiaquilegia

Pi 0.0178

MarkerSeek score 65.60

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 6
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	428-441 bp	1.0	79.5
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	CACATGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	446-459 bp	1.0	78.5
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	390-403 bp	1.0	76.7
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	425-438 bp	1.0	76.3
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	ACCGGTCCATGTCGATTACT	50.0	50.0	57.5	58.5	485-498 bp	1.0	75.3