Hotspot

rps16-trnQ-UUG

LSC · 5794-6928 · 1135 bp

Back to Semiaquilegia

Pi 0.0143
MarkerSeek score 58.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1135
variable_sites
16
pi
0.0143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGAGCACCTCAGAATTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1273-1277 bp 1.0 80.4
rps16-trnQ-UUG_p2 AGAACCCACAACCGATCATG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1230-1234 bp 1.0 80.4
rps16-trnQ-UUG_p3 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1225-1229 bp 1.0 80.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1204-1208 bp 1.0 80.2
rps16-trnQ-UUG_p5 AACCCACAACCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1228-1232 bp 1.0 79.8