Hotspot

trnK-UUU-rps16

LSC · 4040-4732 · 693 bp

Back to Semiaquilegia

Pi 0.0212

MarkerSeek score 75.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 14
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.2	57.9	797-825 bp	1.0	82.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.2	57.9	796-824 bp	1.0	82.9
trnK-UUU-rps16_p3	TCTACCAACGGTATGGACGA	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	57.9	57.9	862-890 bp	1.0	82.8
trnK-UUU-rps16_p4	TCTAGCCGCACTTAAAAGCC	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.3	57.9	811-839 bp	1.0	82.0
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	ATTTCCTTGAAAAAGGCGCG	50.0	45.0	58.3	57.9	806-834 bp	1.0	81.9