Hotspot

rpoC1

Genome · 20918-23715 · 2798 bp

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Pi 0.00386

MarkerSeek score 44.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2798
variable_sites: 24
pi: 0.00386
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCGTCCTAGTTGAATCGGAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.5	58.0	2984-2993 bp	1.0	76.6
rpoC1_p2	CCGTCCTAGTTGAATCGGAG	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.5	57.9	2967-2976 bp	1.0	76.1
rpoC1_p3	CCGTCCTAGTTGAATCGGAG	TTCGATTGCTCGTTCGAGAA	55.0	45.0	57.5	57.7	2989-2998 bp	1.0	74.1
rpoC1_p4	CCGTCCTAGTTGAATCGGAG	TTTCGATTGCTCGTTCGAGA	55.0	45.0	57.5	57.7	2990-2999 bp	1.0	74.1
rpoC1_p5	CCGTCCTAGTTGAATCGGAG	CGATTGCTCGTTCGAGAACT	55.0	50.0	57.5	58.4	2987-2996 bp	1.0	73.8