Hotspot

rpl32-trnL-UAG

Genome · 116374-117292 · 919 bp

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Pi 0.00653

MarkerSeek score 60.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 919
variable_sites: 17
pi: 0.00653
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1041-1057 bp	1.0	82.1
rpl32-trnL-UAG_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1042-1058 bp	1.0	82.1
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.2	58.1	1062-1078 bp	1.0	81.5
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.2	58.1	1064-1080 bp	1.0	81.5
rpl32-trnL-UAG_p5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1051-1067 bp	1.0	81.2