Hotspot

ycf4-cemA

Genome · 61459-62125 · 667 bp

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Pi 0.008

MarkerSeek score 56.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 667
variable_sites: 14
pi: 0.008
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGAACAAAAAGCTGCCGAAT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	798-820 bp	1.0	80.4
ycf4-cemA_p2	AATCGAACAAAAAGCTGCCG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	801-823 bp	1.0	80.3
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	779-801 bp	1.0	80.0
ycf4-cemA_p4	TTGCGTGTACCGATTGAAGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	767-789 bp	1.0	79.6
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	795-817 bp	1.0	79.5