Hotspot

trnT-GGU-psbD

Genome · 32240-33733 · 1494 bp

Back to Semenovia

Pi 0.00745

MarkerSeek score 57.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: Genome
length_bp: 1494
variable_sites: 29
pi: 0.00745
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	AGACCGGACCATCCTACAAA	50.0	50.0	58.3	58.1	1557-1636 bp	1.0	82.8
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GAGCAATAGACCGGACCATC	50.0	55.0	58.3	57.8	1564-1643 bp	1.0	81.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	AGAGCAATAGACCGGACCAT	50.0	50.0	58.3	58.2	1565-1644 bp	1.0	81.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1542-1621 bp	1.0	81.3
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1541-1620 bp	1.0	80.1