Hotspot

rpoB-trnC-GCA

Genome · 26955-28182 · 1228 bp

Back to Semenovia

Pi 0.00519

MarkerSeek score 60.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1228
variable_sites: 18
pi: 0.00519
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1365-1378 bp	1.0	80.2
rpoB-trnC-GCA_p2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1366-1379 bp	1.0	80.1
rpoB-trnC-GCA_p3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1383-1396 bp	1.0	78.2
rpoB-trnC-GCA_p4	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1384-1397 bp	1.0	78.2
rpoB-trnC-GCA_p5	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1347-1360 bp	1.0	78.0