Hotspot

rps16-trnQ-UUG

Genome · 5889-7126 · 1238 bp

Back to Semenovia

Pi 0.008

MarkerSeek score 60.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 1238
variable_sites: 26
pi: 0.008
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGCTTTCTACCACAGCGTTT	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.0	58.5	1211-1345 bp	1.0	81.0
rps16-trnQ-UUG_p2	TTGCTTTCTACCACAGCGTT	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.0	58.5	1212-1346 bp	1.0	81.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1222-1356 bp	1.0	80.2
rps16-trnQ-UUG_p4	TGCTTTCTACCACAGCGTTT	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.0	57.3	1193-1327 bp	1.0	80.0
rps16-trnQ-UUG_p5	TTGCTTTCTACCACAGCGTT	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.0	57.3	1194-1328 bp	1.0	80.0