Hotspot

ycf1

Genome · 88385-93967 · 5583 bp

Back to Selenicereus

Pi 0.00928

MarkerSeek score 34.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5583
variable_sites: 77
pi: 0.00928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 34.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCGCAAAAGATCCTTCTCC	GTAGTTTGTATGGCCAGCGA	50.0	50.0	57.9	58.0	1081-1102 bp	1.0	82.5
ycf1_p2	ACCGCAAAAGATCCTTCTCC	AGTAGTTTGTATGGCCAGCG	50.0	50.0	57.9	58.0	1082-1103 bp	1.0	82.4
ycf1_p3	AGAGGGATTCACCGCAAAAG	GTAGTTTGTATGGCCAGCGA	50.0	50.0	58.2	58.0	1091-1112 bp	1.0	82.0
ycf1_p4	GAAGAGGGATTCACCGCAAA	GTAGTTTGTATGGCCAGCGA	50.0	50.0	58.2	58.0	1093-1114 bp	1.0	81.9
ycf1_p5	GAGGGATTCACCGCAAAAGA	GTAGTTTGTATGGCCAGCGA	50.0	50.0	58.2	58.0	1090-1111 bp	1.0	81.9