Hotspot

rpl22

Genome · 66020-66562 · 543 bp

Back to Selenicereus

Pi 0.0258

MarkerSeek score 52.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: Genome
length_bp: 543
variable_sites: 21
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTTGTGCAAACCAAAGGGAA	AAGTCAAGGGACCATTTGGA	45.0	45.0	57.3	56.6	653 bp	1.0	66.2
rpl22_p2	GTTGTGCAAACCAAAGGGAA	AGTCAAGGGACCATTTGGAT	45.0	45.0	57.3	56.4	652 bp	1.0	64.3
rpl22_p3	GTTGTGCAAACCAAAGGGAA	AAAGTCAAGGGACCATTTGGA	45.0	42.9	57.3	57.4	654 bp	1.0	64.2
rpl22_p4	GTTGTGCAAACCAAAGGGAA	GACCATAAAGTCAAGGGACCA	45.0	47.6	57.3	57.3	660 bp	1.0	63.4
rpl22_p5	GTTGTGCAAACCAAAGGGAA	TGACCATAAAGTCAAGGGACC	45.0	47.6	57.3	57.3	661 bp	1.0	63.4