Hotspot

rps12-psbB

Genome · 54123-55204 · 1082 bp

Back to Selenicereus

Pi 0.00739

MarkerSeek score 63.50

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: Genome
length_bp: 1082
variable_sites: 12
pi: 0.00739
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	ACACCCTAGTACACGTTCCT	GCCGGGATCATTCAATACGA	50.0	50.0	57.7	57.8	1245-1246 bp	1.0	77.1
rps12-psbB_p2	ACACCCTAGTACACGTTCCT	TGGACAGAAATTAACCGGCC	50.0	50.0	57.7	58.2	1262-1263 bp	1.0	77.0
rps12-psbB_p3	GCGGGGGATTTCTTGACATT	GCCGGGATCATTCAATACGA	50.0	50.0	58.5	57.8	1201-1202 bp	1.0	75.3
rps12-psbB_p4	GCGGGGGATTTCTTGACATT	TGGACAGAAATTAACCGGCC	50.0	50.0	58.5	58.2	1218-1219 bp	1.0	75.2
rps12-psbB_p5	ACCCTAGTACACGTTCCTCG	GCCGGGATCATTCAATACGA	55.0	50.0	58.5	57.8	1243-1244 bp	1.0	75.1