Hotspot

ycf3-trnS-GGA

Genome · 30877-31675 · 799 bp

Back to Selenicereus

Pi 0.00584

MarkerSeek score 50.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: Genome
length_bp: 799
variable_sites: 7
pi: 0.00584
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ATTTTCATTCCTTGCCCCGT	47.6	45.0	58.0	58.1	937-938 bp	1.0	71.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	888-889 bp	1.0	71.6
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	909-910 bp	1.0	71.1
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	CATTCCTTGCCCCGTACTTT	47.6	50.0	58.0	58.2	932-933 bp	1.0	71.0
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	TTTCATTCCTTGCCCCGTAC	47.6	50.0	58.0	58.2	935-936 bp	1.0	70.9