Hotspot

ycf1

SSC · 120502-125670 · 5169 bp

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Pi 0.0416
MarkerSeek score 69.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5169
variable_sites
1083
pi
0.0416
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GCGCATAGAGCCCATGATTA AAGAGGGATCCACCGAAGAA 50.0 50.0 57.9 58.0 1118-1148 bp 0.869565 82.7
ycf1_p2 GCGCATAGAGCCCATGATTA ATCGTTTACCTACCCACCCA 50.0 50.0 57.9 58.0 715-724 bp 0.521739 69.4
ycf1_p3 AATCCTCAGACCATTCGGGA ATCGTTTACCTACCCACCCA 50.0 50.0 58.1 58.0 1103-1112 bp 0.434783 65.2
ycf1_p4 ATCCTCAGACCATTCGGGAA ATCGTTTACCTACCCACCCA 50.0 50.0 58.1 58.0 1102-1111 bp 0.434783 65.2
ycf1_p5 ATCCTCAGACCATTCGGGAA AAGAGGGATCCACCGAAGAA 50.0 50.0 58.1 58.0 1505-1514 bp 0.434783 63.9