Hotspot

rpl32-trnL-UAG

SSC · 110837-111325 · 489 bp

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Pi 0.0916
MarkerSeek score 73.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
489
variable_sites
175
pi
0.0916
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TAGGAGGTCACAGTTGGGTT TTCGAACCGAGATGCTCAAG 50.0 50.0 57.9 58.0 978-1025 bp 0.565217 73.7
rpl32-trnL-UAG_p2 TTAGGAGGTCACAGTTGGGT TTCGAACCGAGATGCTCAAG 50.0 50.0 57.9 58.0 979-1026 bp 0.565217 73.7
rpl32-trnL-UAG_p3 TAGGAGGTCACAGTTGGGTT CGAAGTGGCAAATGCTTGAG 50.0 50.0 57.9 58.0 1404-1450 bp 0.565217 73.5
rpl32-trnL-UAG_p4 TAGGAGGTCACAGTTGGGTT CTCGAAGTGGCAAATGCTTG 50.0 50.0 57.9 58.0 1406-1452 bp 0.565217 73.5
rpl32-trnL-UAG_p5 TTAGGAGGTCACAGTTGGGT CGAAGTGGCAAATGCTTGAG 50.0 50.0 57.9 58.0 1405-1451 bp 0.565217 73.5