Hotspot

ndhF-rpl32

SSC · 110063-110677 · 615 bp

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Pi 0.0575
MarkerSeek score 79.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
615
variable_sites
168
pi
0.0575
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAAGGGGAAATTGAGCCGAT TTCGAACCGAGATGCTCAAG 50.0 50.0 57.9 58.0 2010-2100 bp 0.913043 88.0
ndhF-rpl32_p2 AGCAACTGCACCCGAAAATA TTCGAACCGAGATGCTCAAG 45.0 50.0 58.1 58.0 1988-2078 bp 0.869565 86.0
ndhF-rpl32_p3 TAGCAACTGCACCCGAAAAT TTCGAACCGAGATGCTCAAG 45.0 50.0 58.1 58.0 1989-2079 bp 0.869565 86.0
ndhF-rpl32_p4 ATTTAGCAACTGCACCCGAA TTCGAACCGAGATGCTCAAG 45.0 50.0 58.1 58.0 2047-2082 bp 0.391304 60.5
ndhF-rpl32_p5 AGTGGATCGATCAAACAGCC TTCGAACCGAGATGCTCAAG 50.0 50.0 58.0 58.0 1604-1636 bp 0.304348 53.8