Hotspot

psaJ-rpl33

LSC · 65178-65600 · 423 bp

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Pi 0.0472
MarkerSeek score 78.30
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
423
variable_sites
92
pi
0.0472
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 AGATGCGCTGACATTTCCTT ACACTGTTTCGAACACAACTG 45.0 42.9 57.9 57.3 492-525 bp 0.956522 75.4
psaJ-rpl33_p2 ATCGTTTCTTCCCAGATGCG ACACTGTTTCGAACACAACTG 50.0 42.9 58.3 57.3 505-538 bp 0.956522 73.6
psaJ-rpl33_p3 AGATGCGCTGACATTTCCTT CACTGTTTCGAACACAACTGA 45.0 42.9 57.9 57.0 491-524 bp 0.956522 73.2
psaJ-rpl33_p4 AGATGCGCTGACATTTCCTT AACACTGTTTCGAACACAACTG 45.0 40.9 57.9 57.9 493-526 bp 0.956522 72.7
psaJ-rpl33_p5 AGATGCGCTGACATTTCCTT TCGTACATCTTTACTCTTGGCC 45.0 45.5 57.9 57.9 456-481 bp 0.956522 72.3