Hotspot

accD-psaI

LSC · 57455-58017 · 563 bp

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Pi 0.0512
MarkerSeek score 77.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
563
variable_sites
131
pi
0.0512
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CAAACAACGGCGGGATTAAC AAGAGACCCGCGATACCATA 50.0 50.0 58.0 58.0 1612-1733 bp 1.0 91.7
accD-psaI_p2 TAGTGTGTGCTTCTGGAGGA AAGAGACCCGCGATACCATA 50.0 50.0 58.0 58.0 1434-1555 bp 0.956522 90.1
accD-psaI_p3 TAGTGTGTGCTTCTGGAGGA AGAGACCCGCGATACCATAA 50.0 50.0 58.0 58.0 1524-1550 bp 0.347826 56.4
accD-psaI_p4 GCTACCAAGAGCTTTCTCCC AGAGACCCGCGATACCATAA 55.0 50.0 58.0 58.0 1552-1578 bp 0.347826 56.1
accD-psaI_p5 CAAACAACGGCGGGATTAAC AGAGACCCGCGATACCATAA 50.0 50.0 58.0 58.0 1702-1728 bp 0.347826 56.0