Hotspot

ndhC-trnV-UAC

LSC · 49299-50191 · 893 bp

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Pi 0.0449
MarkerSeek score 80.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
893
variable_sites
201
pi
0.0449
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 907-950 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 906-949 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATCATATTCATAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 906-949 bp 1.0 52.0
ndhC-trnV-UAC_p4 TCATATTCATAAAGCAGAAACAT GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 905-948 bp 1.0 52.0
ndhC-trnV-UAC_p5 ATCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.9 57.8 907-950 bp 1.0 52.0