Hotspot

trnG-UCC-trnfM-CAU

LSC · 36179-36324 · 146 bp

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Pi 0.0488
MarkerSeek score 80.00
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
146
variable_sites
33
pi
0.0488
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 214-234 bp 1.0 90.3
trnG-UCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 208-228 bp 1.0 89.8
trnG-UCC-trnfM-CAU_p3 AAGAACGAAAAACGCGGATG CTTGAGGTCACGGGTTCAAA 45.0 50.0 57.7 58.0 260-280 bp 0.913043 85.7
trnG-UCC-trnfM-CAU_p4 CAAGAACGAAAAACGCGGAT CTTGAGGTCACGGGTTCAAA 45.0 50.0 57.7 58.0 261-281 bp 0.869565 84.0
trnG-UCC-trnfM-CAU_p5 ATCAAGAACGAAAAACGCGG CTTGAGGTCACGGGTTCAAA 45.0 50.0 57.7 58.0 263-283 bp 0.826087 82.3