Hotspot

lhbA-trnG-UCC

LSC · 35331-36107 · 777 bp

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Pi 0.07
MarkerSeek score 79.50
Primer available yes
Label lhbA-trnG-UCC

Feature Metadata

label_name
lhbA-trnG-UCC
feature_type
igs
region
LSC
length_bp
777
variable_sites
221
pi
0.07
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-UCC_p1 TGGTGGGGATCCTTAATTCTC CGTCTTCTCCTTGGCAAAGA 47.6 50.0 56.8 57.8 664-856 bp 0.956522 71.0
lhbA-trnG-UCC_p2 TGGTGGGGATCCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 45.5 50.0 58.3 57.8 664-856 bp 0.956522 70.9
lhbA-trnG-UCC_p3 TTGGTGGGGATCCTTAATTCTC CGTCTTCTCCTTGGCAAAGA 45.5 50.0 57.5 57.8 665-857 bp 0.956522 68.7
lhbA-trnG-UCC_p4 GGTGGGGATCCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 56.5 57.8 663-855 bp 0.956522 68.7
lhbA-trnG-UCC_p5 GGTGGGGATCCTTAATTCTCTC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.6 57.8 663-855 bp 0.913043 68.3