Hotspot

rpoB-trnC-GCA

LSC · 26820-27964 · 1145 bp

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Pi 0.0467
MarkerSeek score 80.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1145
variable_sites
247
pi
0.0467
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TTCCCTCATTTCCATCCCGG CCGGATTTGAACTGGGGAAA 55.0 50.0 59.4 58.1 1141-1246 bp 1.0 79.8
rpoB-trnC-GCA_p2 TTCCCTCATTTCCATCCCGG AAAAGGATTTGCAGTCCCCC 55.0 50.0 59.4 58.4 1123-1228 bp 1.0 77.6
rpoB-trnC-GCA_p3 TTCCCTCATTTCCATCCCGG AGTCTTGTGTTGATCAGGCG 55.0 50.0 59.4 58.2 1165-1270 bp 0.956522 77.1
rpoB-trnC-GCA_p4 TTCCCTCATTTCCATCCCGG GTCTTGTGTTGATCAGGCGA 55.0 50.0 59.4 58.2 1164-1269 bp 0.956522 77.0
rpoB-trnC-GCA_p5 TTCCCTCATTTCCATCCCGG TCTTGTGTTGATCAGGCGAC 55.0 50.0 59.4 58.2 1163-1268 bp 0.956522 77.0