Hotspot

ndhA

Genome · 111750-113867 · 2118 bp

Pi 0.00112

MarkerSeek score 47.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2118
variable_sites: 5
pi: 0.00112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2259 bp	1.0	70.5
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2257 bp	1.0	70.0
ndhA_p3	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2258 bp	1.0	64.9
ndhA_p4	ACCCAGTTACCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2259 bp	1.0	64.8
ndhA_p5	CCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	50.0	50.0	56.2	57.8	2256 bp	1.0	64.3