Hotspot

matK

Genome · 1670-3208 · 1539 bp

Pi 0.000217

MarkerSeek score 46.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1539
variable_sites: 1
pi: 0.000217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAACCCTTTCTTGTTTCTT	CAACACCCTGTTCTGACCAT	35.0	50.0	52.8	57.7	1663 bp	1.0	44.0
matK_p2	TCAACCCTTTCTTGTTTCTT	AATGCAACACCCTGTTCTGA	35.0	45.0	52.8	57.6	1667 bp	1.0	44.0
matK_p3	TCAACCCTTTCTTGTTTCTT	ATGCAACACCCTGTTCTGAC	35.0	50.0	52.8	58.4	1666 bp	1.0	44.0
matK_p4	ACCCTTTCTTGTTTCTTAATTTCGT	CAACACCCTGTTCTGACCAT	32.0	50.0	57.7	57.7	1660 bp	1.0	44.0
matK_p5	ACCCTTTCTTGTTTCTTAATTTCGT	AATGCAACACCCTGTTCTGA	32.0	45.0	57.7	57.6	1664 bp	1.0	44.0