Hotspot

atpF

Genome · 32962-34344 · 1383 bp

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Pi 0.00062
MarkerSeek score 48.60
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
Genome
length_bp
1383
variable_sites
3
pi
0.00062
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 AAAAAGGGCGAGCGAAGTAA TTCGGAAGCAAGGGGAAAAA 45.0 45.0 58.1 57.9 1525-1533 bp 1.0 78.7
atpF_p2 AAAAAGGGCGAGCGAAGTAA TTTCGGAAGCAAGGGGAAAA 45.0 45.0 58.1 57.9 1526-1534 bp 1.0 78.7
atpF_p3 AAAAAGGGCGAGCGAAGTAA TTTTCGGAAGCAAGGGGAAA 45.0 45.0 58.1 57.9 1527-1535 bp 1.0 78.7
atpF_p4 AAAAAGGGCGAGCGAAGTAA TTTTTCGGAAGCAAGGGGAA 45.0 45.0 58.1 57.9 1528-1536 bp 1.0 78.7
atpF_p5 AAAAAGGGCGAGCGAAGTAA TCGAAGGGTTGCCATTAGTG 45.0 50.0 58.1 57.9 1560-1568 bp 1.0 78.4