Hotspot

ndhF-rpl32

Genome · 104350-105263 · 914 bp

Back to Secale

Pi 0.00198
MarkerSeek score 39.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
Genome
length_bp
914
variable_sites
5
pi
0.00198
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1025-1053 bp 1.0 40.5
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 998-1026 bp 1.0 40.5
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 999-1027 bp 1.0 40.5
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 1000-1028 bp 1.0 40.5
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 1001-1029 bp 1.0 40.5