Hotspot

petA-psbJ

Genome · 60949-61776 · 828 bp

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Pi 0.0015
MarkerSeek score 36.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
828
variable_sites
3
pi
0.0015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC GCTGGTATTGCTGTGATTGG 50.0 50.0 57.9 57.1 1005-1006 bp 1.0 73.0
petA-psbJ_p2 TGTTCTTCTTCGCATCCGTT GCTGGTATTGCTGTGATTGG 45.0 50.0 57.8 57.1 998-999 bp 1.0 72.1
petA-psbJ_p3 TTGTTCTTCTTCGCATCCGT GCTGGTATTGCTGTGATTGG 45.0 50.0 57.8 57.1 999-1000 bp 1.0 72.1
petA-psbJ_p4 GGCCTTTTGTTCTTCTTCGC ACTGTAGCTGGTATTGCTGTG 50.0 47.6 57.9 58.0 1011-1012 bp 1.0 71.6
petA-psbJ_p5 TGTTCTTCTTCGCATCCGTT ACTGTAGCTGGTATTGCTGTG 45.0 47.6 57.8 58.0 1004-1005 bp 1.0 70.8