Hotspot

rbcL-psaI

Genome · 56220-57583 · 1364 bp

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Pi 0.00189
MarkerSeek score 46.70
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
Genome
length_bp
1364
variable_sites
5
pi
0.00189
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CGCAGCTTGTGAAGTATGGA GTCATTGCAATTGCCGGAAA 50.0 45.0 58.3 57.9 1499-1788 bp 1.0 77.5
rbcL-psaI_p2 AGTTCGAGCCGGTAGATACT GTCATTGCAATTGCCGGAAA 50.0 45.0 57.7 57.9 1464-1753 bp 1.0 76.9
rbcL-psaI_p3 GGCGATCAAATTCGAGTTCG GTCATTGCAATTGCCGGAAA 50.0 45.0 57.6 57.9 1478-1767 bp 1.0 76.8
rbcL-psaI_p4 TGGAAGGCGATCAAATTCGA GTCATTGCAATTGCCGGAAA 45.0 45.0 57.6 57.9 1483-1772 bp 1.0 76.5
rbcL-psaI_p5 AGCTTGCAAATGGAGTCCTG GTCATTGCAATTGCCGGAAA 50.0 45.0 58.5 57.9 1526-1815 bp 1.0 76.0