Hotspot

trnS-GCU-psbD

Genome · 7643-8703 · 1061 bp

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Pi 0.00197
MarkerSeek score 40.10
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
Genome
length_bp
1061
variable_sites
4
pi
0.00197
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA GAGCAATAGGCCAGACCATC 45.0 55.0 58.0 58.1 1215-1218 bp 1.0 79.2
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1193-1196 bp 1.0 78.1
trnS-GCU-psbD_p3 GAGCGGAAAGAGAGGGATTC GAGCAATAGGCCAGACCATC 55.0 55.0 57.8 58.1 1266-1269 bp 1.0 77.6
trnS-GCU-psbD_p4 ATTAGCAATCCGCCGCTTTA CCCTTCGTAACCAGTCATCC 45.0 55.0 58.0 57.7 1177-1180 bp 1.0 77.6
trnS-GCU-psbD_p5 ATTAGCAATCCGCCGCTTTA AGGCCAGACCATCCTACAAA 45.0 50.0 58.0 58.3 1208-1211 bp 1.0 77.4