Hotspot

matK-trnK-UUU

Genome · 3209-3893 · 685 bp

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Pi 0.000876
MarkerSeek score 47.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
Genome
length_bp
685
variable_sites
1
pi
0.000876
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCATAATCGTGGGCAAATGC AATGTTTTGACAGAAGCGCG 50.0 45.0 57.9 58.0 898-904 bp 1.0 82.7
matK-trnK-UUU_p2 CATAATCGTGGGCAAATGCA AATGTTTTGACAGAAGCGCG 45.0 45.0 57.1 58.0 897-903 bp 1.0 76.9
matK-trnK-UUU_p3 CCATAATCGTGGGCAAATGC TGGGTTGCTAACTCAATGGT 50.0 45.0 57.9 57.0 822-828 bp 1.0 75.2
matK-trnK-UUU_p4 CCATAATCGTGGGCAAATGC TCAATGTTTTGACAGAAGCGC 50.0 42.9 57.9 58.0 900-906 bp 1.0 75.0
matK-trnK-UUU_p5 CCATAATCGTGGGCAAATGC TCTGGGTTGCTAACTCAATGG 50.0 47.6 57.9 57.9 824-830 bp 1.0 74.4