Hotspot

ycf46

LSC · 78777-80252 · 1476 bp

Back to Scytosiphon

Pi 0.00203

MarkerSeek score 59.90

Primer available yes

Label ycf46

Feature Metadata

label_name: ycf46
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 3
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	TCGTCGTCGAAGAAAAAGAA	TGCTAGGAATAATTTTAGACCAAGA	40.0	32.0	55.4	56.0	1599 bp	1.0	40.2
ycf46_p2	CGTCGTCGAAGAAAAAGAAAGA	TGCTAGGAATAATTTTAGACCAAGA	40.9	32.0	57.3	56.0	1598 bp	1.0	40.2
ycf46_p3	TCGTCGTCGAAGAAAAAGAAAG	TGCTAGGAATAATTTTAGACCAAGA	40.9	32.0	57.3	56.0	1599 bp	1.0	40.2
ycf46_p4	TCGTCGTCGAAGAAAAAGAAA	TGCTAGGAATAATTTTAGACCAAGA	38.1	32.0	56.2	56.0	1599 bp	1.0	40.2
ycf46_p5	TCGTCGTCGAAGAAAAAGAA	TGCTAGGAATAATTTTAGACCAAG	40.0	33.3	55.4	54.7	1599 bp	1.0	40.2