Hotspot

rpl4

SSC · 49766-50536 · 771 bp

Back to Scytosiphon

Pi 0.00259

MarkerSeek score 60.20

Primer available yes

Label rpl4

Feature Metadata

label_name: rpl4
feature_type: gene
region: SSC
length_bp: 771
variable_sites: 2
pi: 0.00259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl4_p1	AGCCTAGGGTCTACTAAGAATGT	AAGAAAGTGAAGAGACCGCG	43.5	50.0	57.9	58.2	905 bp	1.0	53.3
rpl4_p2	AGCCTAGGGTCTACTAAGAATGT	GAAAGTGAAGAGACCGCGAA	43.5	50.0	57.9	58.2	903 bp	1.0	53.2
rpl4_p3	AGCCTAGGGTCTACTAAGAATGT	AAGTGAAGAGACCGCGAATG	43.5	50.0	57.9	58.3	901 bp	1.0	52.7
rpl4_p4	AGCCTAGGGTCTACTAAGAATGT	AAAGTGAAGAGACCGCGAAT	43.5	45.0	57.9	57.5	902 bp	1.0	51.4
rpl4_p5	GCCTAGGGTCTACTAAGAATGT	AAGAAAGTGAAGAGACCGCG	45.5	50.0	56.4	58.2	904 bp	1.0	49.9