Hotspot

petA

LSC · 115278-116225 · 948 bp

Back to Scytosiphon

Pi 0.00211

MarkerSeek score 60.00

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 948
variable_sites: 2
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ACTTGTTTCTATTAGTGCAAGCT	ACCTCAATTAATCTAATAAACAAGAGC	34.8	29.6	56.5	56.3	1162-1163 bp	1.0	40.1
petA_p2	ACTTGTTTCTATTAGTGCAAGCT	CCTCAATTAATCTAATAAACAAGAGCT	34.8	29.6	56.5	56.0	1161-1162 bp	1.0	40.1
petA_p3	ACTTGTTTCTATTAGTGCAAGCTT	ACCTCAATTAATCTAATAAACAAGAGC	33.3	29.6	57.1	56.3	1162-1163 bp	1.0	40.1
petA_p4	ACTTGTTTCTATTAGTGCAAGC	ACCTCAATTAATCTAATAAACAAGAGC	36.4	29.6	55.0	56.3	1162-1163 bp	1.0	40.1
petA_p5	ACTTGTTTCTATTAGTGCAAGCT	CCTCAATTAATCTAATAAACAAGAGC	34.8	30.8	56.5	54.8	1161-1162 bp	1.0	40.1