Hotspot

chlL

LSC · 110158-111027 · 870 bp

Back to Scytosiphon

Pi 0.0023

MarkerSeek score 49.10

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: LSC
length_bp: 870
variable_sites: 2
pi: 0.0023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	GGGGTACGAAAGGCTAGTTG	CGCGGTTCAGCAAAAATCAT	55.0	45.0	58.0	58.0	1434-1437 bp	1.0	80.2
chlL_p2	GGGTACGAAAGGCTAGTTGG	CGCGGTTCAGCAAAAATCAT	55.0	45.0	58.0	58.0	1433-1436 bp	1.0	80.2
chlL_p3	TAGTTCGTTGGCGTGACAAA	CGCGGTTCAGCAAAAATCAT	45.0	45.0	58.1	58.0	1933-1936 bp	1.0	79.8
chlL_p4	TTAGTTCGTTGGCGTGACAA	CGCGGTTCAGCAAAAATCAT	45.0	45.0	58.1	58.0	1934-1937 bp	1.0	79.8
chlL_p5	TTTAGTTCGTTGGCGTGACA	CGCGGTTCAGCAAAAATCAT	45.0	45.0	58.1	58.0	1935-1938 bp	1.0	79.8