Hotspot

atpA

LSC · 99709-101214 · 1506 bp

Back to Scytosiphon

Pi 0.00199

MarkerSeek score 59.90

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 3
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TCTGTAATTCGGTTGTTTTCT	TTATTTAAGGCCTCTCTAAAGT	33.3	31.8	53.4	52.2	1670 bp	1.0	40.1
atpA_p2	TCTGTAATTCGGTTGTTTTCT	TTTAAGGCCTCTCTAAAGTATT	33.3	31.8	53.4	52.2	1667 bp	1.0	40.1
atpA_p3	TCTGTAATTCGGTTGTTTTCTT	TTATTTAAGGCCTCTCTAAAGT	31.8	31.8	54.2	52.2	1670 bp	1.0	40.1
atpA_p4	TCTGTAATTCGGTTGTTTTCTT	TTTAAGGCCTCTCTAAAGTATT	31.8	31.8	54.2	52.2	1667 bp	1.0	40.1
atpA_p5	TTCTGTAATTCGGTTGTTTTCT	TTTAAGGCCTCTCTAAAGTATT	31.8	31.8	54.2	52.2	1668 bp	1.0	40.1