Hotspot

trnC-psbY

LSC · 129540-130298 · 759 bp

Back to Scytosiphon

Pi 0.0105

MarkerSeek score 55.20

Primer available yes

Label trnC-psbY

Feature Metadata

label_name: trnC-psbY
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 8
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-psbY_p1	AGTTCGAATCTGAGTGTCGC	TGCAGCTTCATGGGCATTAT	50.0	45.0	58.0	57.9	855-856 bp	1.0	80.0
trnC-psbY_p2	TAAGGCCGTGGATTGCAAAT	TGCAGCTTCATGGGCATTAT	45.0	45.0	58.2	57.9	886-887 bp	1.0	78.9
trnC-psbY_p3	CCGTGGATTGCAAATCCTCT	TGCAGCTTCATGGGCATTAT	50.0	45.0	58.2	57.9	881-882 bp	1.0	78.2
trnC-psbY_p4	TCGAATCTGAGTGTCGCCTA	TGCAGCTTCATGGGCATTAT	50.0	45.0	58.3	57.9	852-853 bp	1.0	78.1
trnC-psbY_p5	AGTTCGAATCTGAGTGTCGC	CTGCAGCTTCATGGGCATTA	50.0	50.0	58.0	58.6	856-857 bp	1.0	75.8