Hotspot

trnI-rns

IRa · 55096-55206 · 111 bp

Back to Scytosiphon

Pi 0.018

MarkerSeek score 62.90

Primer available yes

Label trnI-rns

Feature Metadata

label_name: trnI-rns
feature_type: igs
region: IRa
length_bp: 111
variable_sites: 2
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-rns_p1	GCCATCCTGGATTTGAACCA	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.1	57.9	240-249 bp	1.0	78.9
trnI-rns_p2	GCCATCCTGGATTTGAACCA	GTAACAAGGTAGCCGTACGG	50.0	55.0	58.1	58.1	225-234 bp	1.0	78.8
trnI-rns_p3	GCCATCCTGGATTTGAACCA	AACCTTTTTGGAGAGAGGCG	50.0	50.0	58.1	58.1	277-286 bp	1.0	78.6
trnI-rns_p4	AATGGGCCATCCTGGATTTG	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.2	57.9	245-254 bp	1.0	78.4
trnI-rns_p5	CAAATGGGCCATCCTGGATT	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.2	57.9	247-256 bp	1.0	78.4