Hotspot

rps17-rpl29

SSC · 46476-46530 · 55 bp

Back to Scytosiphon

Pi 0.0364
MarkerSeek score 73.40
Primer available yes
Label rps17-rpl29

Feature Metadata

label_name
rps17-rpl29
feature_type
igs
region
SSC
length_bp
55
variable_sites
2
pi
0.0364
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps17-rpl29_p1 TCAACAGCAACTACAACGCT CGTTTACGACGTGGAACAAA 45.0 45.0 58.1 57.0 228 bp 1.0 72.3
rps17-rpl29_p2 TCAACAGCAACTACAACGCT CGTTTACGACGTGGAACAAAA 45.0 42.9 58.1 57.7 228 bp 1.0 69.9
rps17-rpl29_p3 TCAACAGCAACTACAACGCT ACGACGTGGAACAAAACAATC 45.0 42.9 58.1 57.7 223 bp 1.0 69.7
rps17-rpl29_p4 TCAACAGCAACTACAACGCT ACGACGTGGAACAAAACAAT 45.0 40.0 58.1 56.5 223 bp 1.0 68.3
rps17-rpl29_p5 TCAACAGCAACTACAACGCT CGACGTGGAACAAAACAATCT 45.0 42.9 58.1 57.4 222 bp 1.0 67.5