Hotspot

rpl32-trnL-UAG

SSC · 111922-112367 · 446 bp

Back to Scutellaria

Pi 0.0422

MarkerSeek score 73.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 156
pi: 0.0422
unique_haplotype_count: 133
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTACGACAAACGAATAAACT	TCCTAAGAGCAGCGTGTCTA	33.3	50.0	53.6	57.9	334-699 bp	1.0	52.0
rpl32-trnL-UAG_p2	TGTACGACAAACGAATAAACT	AGCAGCGTGTCTACCAATTT	33.3	45.0	53.6	57.8	327-692 bp	1.0	52.0
rpl32-trnL-UAG_p3	TGTACGACAAACGAATAAACT	CCTAAGAGCAGCGTGTCTAC	33.3	55.0	53.6	57.8	333-698 bp	1.0	52.0
rpl32-trnL-UAG_p4	TGTACGACAAACGAATAAACT	CTAAGAGCAGCGTGTCTACC	33.3	55.0	53.6	57.8	332-697 bp	1.0	52.0
rpl32-trnL-UAG_p5	TGTACGACAAACGAATAAACT	GAGCAGCGTGTCTACCAATT	33.3	50.0	53.6	58.3	328-693 bp	1.0	52.0