Hotspot

rpl20-rps12

LSC · 69147-69940 · 794 bp

Back to Scutellaria

Pi 0.013

MarkerSeek score 67.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 131
pi: 0.013
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGACCCCCGAAAAC	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	952-975 bp	0.994382	88.4
rpl20-rps12_p2	ATGAGACCCCCGAAAACTTG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	949-972 bp	0.994382	88.3
rpl20-rps12_p3	TGAATGAGACCCCCGAAAAC	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	957-980 bp	1.0	88.0
rpl20-rps12_p4	ATGAGACCCCCGAAAACTTG	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	954-977 bp	1.0	88.0
rpl20-rps12_p5	CGGGCTATATATCCGCGTTT	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.7	57.8	897-920 bp	0.994382	87.8