Hotspot

petA-psbJ

LSC · 63478-64484 · 1007 bp

Back to Scutellaria

Pi 0.0284

MarkerSeek score 72.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 240
pi: 0.0284
unique_haplotype_count: 134
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	821-1124 bp	1.0	86.7
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	820-1123 bp	1.0	86.2
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	820-1123 bp	1.0	85.8
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	823-1126 bp	0.926966	83.7
petA-psbJ_p5	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	822-1125 bp	0.926966	83.2