Hotspot

rbcL-accD

LSC · 56965-57677 · 713 bp

Back to Scutellaria

Pi 0.0205

MarkerSeek score 62.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 129
pi: 0.0205
unique_haplotype_count: 103
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	ACTATTCTTCGCGTGGGTTC	50.0	50.0	58.0	58.0	2030-2088 bp	0.983146	91.1
rbcL-accD_p2	TCATGGTATGCACTTCCGTG	ACTATTCTTCGCGTGGGTTC	50.0	50.0	58.0	58.0	1830-1888 bp	0.983146	91.1
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	ACTATTCTTCGCGTGGGTTC	55.0	50.0	58.0	58.0	1409-1467 bp	0.983146	91.0
rbcL-accD_p4	TCTTCACATTCACCGTGCAA	ACTATTCTTCGCGTGGGTTC	45.0	50.0	58.0	58.0	1878-1936 bp	0.983146	91.0
rbcL-accD_p5	TTCTTCACATTCACCGTGCA	ACTATTCTTCGCGTGGGTTC	45.0	50.0	58.0	58.0	1879-1937 bp	0.983146	91.0